The French federation did not give any explanation for their absence, but sports daily L'Equipe reported that Upamecano had a sore throat and Tchouameni was suffering from a knock.
France will play Morocco in the semi-finals of the World Cup on December 14 at Al Bayt Stadium.
France and Morocco have met five times in official international fixtures.
The first took place in 1988, which France won 2-1, but their history goes back further. They played unofficial games in the 1960s, 70s and 80s.
France have never lost an official match against Morocco. The most recent clash was a 2-2 draw in Paris in 2007.