This is Klaassen's first Paralympic gold medal, having already won a silver medal five years ago at Rio 2016 in the same event along with then pilot partner Haliegh Dolman.
A month before she even stood on the track, Klaassen already had an inkling that there might be a possibility to win gold.
Klaassen clocked in a time of 1:05.291 which secured her for the top spot on the podium but also broke the Paralympic record of 1:06.283 set by Sophie Thornhill at Rio 2016.
Klaassen, who switched from gymnastics to take up para cycling after watching Dutch Kim van Dijk cyclist competing in 2012, had always dreamt to become a champion.
This is the second gold medal for the Netherlands at the Tokyo 2020 Paralympic Games after fellow cyclist Tristan Bangma won in the men's B 4,000m individual pursuit where he also broke the World and Paralympic record.
Silver went to Great Britain's Aileen McGlynn (Pilot: Helen Scott) with Belgium's Griet Hoet (Pilot: Anneleen Monsiuer).